Green Fluorescent Protein Transformation

Bio Tech June

Our second workshop was an exciting adventure in genetic engineering!  Our participants were able to take genes originally from sea jellies that allow them to glow, and put them into bacteria!  Not only was the gene for glowing introduced, but also a gene to allow resistance to an antibiotic to allow us to select for only the bacteria that have the glowing gene.  These techniques have allowed scientists to do many different kinds of experiments and make great advances in developmental biology, neurobiology, and much more.  Similar techniques are currently used in agriculture for pesticide resistance among other things. Since the bacteria needed some time to grow, participants could come back the next day to see how well the bacteria they cultured survived and to check for fluorescence using a UV light!

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